CPC C12Q 1/6823 (2013.01) [C12N 5/0602 (2013.01); C12Q 1/6869 (2013.01); C12Q 2563/185 (2013.01)] | 18 Claims |
1. A method for identifying a candidate therapeutic moiety comprising:
(1) administering to an animal or an organoid a library of expression cassettes comprising:
(a) a plurality of nucleic acid sequences, each encoding a different therapeutic moiety operably linked to a therapeutic moiety barcode; and
(b) a plurality of nucleic acid sequences encoding one or more reporter constructs that alone or collectively are indicative of a cell state or a likelihood of a cell state of the cell;
(2) removing a tissue from said animal or organoid and placing a section of said tissue on a coated surface; wherein said surface coating comprises primers encoding a spatial barcode providing coordinates for each of various spots on said coated surface;
(3) detecting a change in a cell state or likelihood of a cell state of one or more cells of the animal or the organoid and coordinating the presence of said therapeutic moiety to a specific cell or location within said tissue section using said spatial barcode,
wherein detecting comprises performing spatial transcriptomic RNA sequencing of a population of cells to determine an amount of the candidate therapeutic moiety present in the population of cells, thereby identifying a candidate therapeutic moiety.
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