CPC C12P 19/305 (2013.01) [C12N 1/20 (2013.01); C12N 9/1205 (2013.01); C12N 9/1241 (2013.01); C12N 15/70 (2013.01); C12Y 207/01032 (2013.01); C12Y 207/07015 (2013.01)] | 3 Claims |
1. A genetically modified Escherichia coli for producing citicoline comprising the following modifications:
a. enzymes involved in reuse of citicoline, or choline, or phosphocholine being disrupted, wherein the enzymes comprise cytidine-5′-diphosphoinositol hydrolase, cytidine-5′-diphosphate-diacylglycerol pyrophosphatase, choline dehydrogenase, alkaline phosphatase or acid phosphatase;
b. choline kinase for catalyzing choline chloride to generate phosphocholine being overexpressed, wherein the choline kinase comprises CKI1 or EKI1 obtained from Saccharomyces cerevisiae, or LicC obtained from Streptococcus;
c. cytidylyltransferase for catalyzing phosphocholine to generate citicoline being overexpressed, wherein the cytidylyltransferase comprises PCT1, CDS1, or ECT1 obtained from Saccharomyces cerevisiae; or CdsA, IspD, MocA, KdsB, or Cca obtained from Escherichia coli; or LicC obtained from Streptococcus; or CD36_40620 obtained from Candida dubliniensis;
d. choline transporter protein, for transporting choline chloride into a cell being overexpressed, wherein the choline transporter protein comprises BetT obtained from Escherichia coli; and
e. uridine-5′-monophosphate phosphorylase for degrading uridine-5′-monophosphate into uridine is disrupted; and the uridine-5′-monophosphate phosphorylase comprises UmpH, UmpG, PhoA, AphA, or YjjG.
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