CPC C07C 233/54 (2013.01) [A61K 39/0013 (2013.01); A61K 39/385 (2013.01); A61P 25/30 (2018.01); A61K 2039/55505 (2013.01); A61K 2039/55555 (2013.01); A61K 2039/55561 (2013.01); A61K 2039/6012 (2013.01); A61K 2039/6037 (2013.01); A61K 2039/627 (2013.01)] | 9 Claims |
1. A method of stimulating an immune response to methamphetamine, comprising:
immunizing an animal by administering to the animal a conjugate comprising one or more compounds having a general formula:
wherein
R1 is —(O—CH2—CH2)p—, wherein p is 1 to 6, or R1 is an optionally substituted C1-6 alkyl, wherein substitution(s) are independently selected from the group consisting of —NO2, —NH2, —OH, ═O, —COOR′ where R′ is H or lower alkyl, —CH2OH, and —CONH2,
R2 is peptide of m amino acid residues, an alkylene oxide of m alkylene oxide monomers, or (CH2)m, where m=2 to 6; and
R3 is selected from the group consisting of a protected or unprotected maleimide, alkyl halide, aryl halide, alpha-haloacyl, pyridyl disulfide, aldehyde, ketone, carboxyl, thiol, thioester, disulfide, N-hydroxy-succinimide, or cyclic thiolactone,
or salts thereof, wherein the one or more compounds or salts thereof are covalently bound through R3 on the compound(s) to a corresponding coupling site on a protein, polypeptide, detectable label, nucleic acid, or solid phase to provide the conjugate.
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