| US 7,582,733 B2 | ||
| Humanized antibodies that recognize beta amyloid peptide | ||
| Guriq Basi, Palo Alto, Calif. (US); and Jose William Saldanha, Enfield (United Kingdom) | ||
| Assigned to Elan Pharma International Limited, County Westmeath (Ireland); and Wyeth, Madison, N.J. (US) | ||
| Filed on Aug. 30, 2002, as Appl. No. 10/232,030. | ||
| Application 10/232030 is a continuation of application No. 10/010942, filed on Dec. 06, 2001, granted, now 7,189,819. | ||
| Application 10/010942 is a continuation in part of application No. 09/723713, filed on Nov. 27, 2000, abandoned. | ||
| Application 09/723713 is a continuation of application No. 09/322289, filed on May 28, 1999. | ||
| Application 09/322289 is a continuation in part of application No. 09/201430, filed on Nov. 30, 1998, granted, now 6,787,523. | ||
| Claims priority of provisional application 60/251892, filed on Dec. 06, 2000. | ||
| Prior Publication US 2005/0009150 A1, Jan. 13, 2005 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. C07K 16/00 (2006.01) | ||
| U.S. Cl. 530—387.1 [530/388.24] | 13 Claims |
| 1. A humanized antibody; which specifically binds to beta amyloid peptide (Aβ) with a binding affinity of at least 107 M−1, or antigen binding fragment thereof selected from the group consisting of a Fab, a Fab′, F(ab′)2, a FV, and a single-chain antibody, wherein the humanized antibody comprises:
(i) a humanized light chain comprising three complementarity determining regions (CDR1, CDR2, and CDR3) from the 3D6 immunoglobulin
light chain variable region sequence set forth as SEQ ID NO:2, and a light chain variable region framework sequence, wherein
at least three framework positions selected from the group consisting of L1, L2, L36, and L46 (Kabat numbering convention)
are occupied by Y, V, L and R, respectively, and
(ii) a humanized light chain comprising three comnplementarity determining regions (CDR1, CDR2, and CDR3) from the 2D6 immunoglobulin
heavy chain variable region sequence set forth as SEQ ID NO:4, and a heavy chain variable regions framework sequence, wherein
framework positions H49, H93, and H94 (Kabat numbering convention) are occupied by A, V, and R respectively.
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