	US 7,582,476 B2
	Artificial cell comprising mutant estrogen receptor
Koichi Saito, Takarazuka (Japan); Norihisa Ohe, Nara (Japan); and Hideo Satoh, Ibaraki (Japan)
Assigned to Sumitomo Chemical Company, Limited, Osaka (Japan)
Appl. No. 10/148,835 PCT Filed Dec. 01, 2000, PCT No. PCT/JP00/08553 § 371(c)(1), (2), (4) Date Oct. 11, 2002, PCT Pub. No. WO01/42307, PCT Pub. Date Jun. 14, 2001.
Claims priority of application No. 11/348022 (JP), filed on Dec. 07, 1999; application No. 11/370667 (JP), filed on Dec. 27, 1999; application No. 2000-207011 (JP), filed on Jul. 07, 2000; application No. 2000-220508 (JP), filed on Jul. 21, 2000; application No. 2000-234053 (JP), filed on Aug. 02, 2000; application No. 2000-235460 (JP), filed on Aug. 03, 2000; application No. 2000-235461 (JP), filed on Aug. 03, 2000; and application No. 2000-235463 (JP), filed on Aug. 03, 2000.
Prior Publication US 2003/0207380 A1, Nov. 06, 2003
Int. Cl. C12N 5/10 (2006.01); C12N 15/11 (2006.01)

U.S. Cl. 435—325 [435/69.1]

26 Claims

1. An artificial cell comprising: (i) a chromosome which comprises a reporter polynucleotide, wherein the reporter polynucleotide comprises an ERE, a TATA sequence and a reporter sequence heterologous to the ERE; and

(ii) a mutant ERα which has an activity for transactivation of the reporter polynucleotide,

wherein in the presence of a partial anti-estrogen and E2 the activity is higher than that of ERα encoded by SEQ ID NO:1 in the presence of the partial anti-estrogen and E2, or in the presence of a partial anti-estrogen the activity is higher than that of ERα encoded by SEQ ID NO:1 in the presence of the partial anti-estrogen, and

wherein the mutant ERα has an amino acid sequence of an ERα comprising one or more substituted amino acids at one or more relative positions selected from 303, 309, 390, 396, 494 and 578, or two or more substituted amino acids at two or more relative positions selected from 303, 309, 390, 396, 415, 494, 531 and 578, wherein the relative positions are based on a homology alignment to an amino acid sequence shown in SEQ ID NO:1.