	US 7,582,259 B2
	Blood analysis device and blood analysis method
Hiroki Ogawa, Yokohama (Japan); and Yasuhiro Horiike, Nishitokyo (Japan)
Assigned to Japan Science and Technology Agency, Saitama (Japan)
Appl. No. 10/546,447 PCT Filed Feb. 18, 2004, PCT No. PCT/JP2004/001802 § 371(c)(1), (2), (4) Date Aug. 19, 2005, PCT Pub. No. WO2004/074846, PCT Pub. Date Sep. 02, 2004.
Claims priority of application No. 2003-040481 (JP), filed on Feb. 19, 2003.
Prior Publication US 2006/0078873 A1, Apr. 13, 2006
Int. Cl. G01N 33/72 (2006.01); C12Q 1/00 (2006.01); C12M 1/34 (2006.01)

U.S. Cl. 422—72 [435/287.1; 435/4; 436/66]

19 Claims

1. A blood analysis device which separates a plasma from a whole blood sample by centrifugation and analyzes a component to be analyzed in blood liquid components, comprising:

(a) a substrate comprising a sensor for analyzing the component to be analyzed in the blood liquid components;

(b) a plasma separating section formed in said substrate, and having a sensor groove for housing the sensor, the plasma being separated in the sensor groove when a centrifugal force is applied to the substrate in a first centrifugal direction;

(c) a blood introducing channel for communicating with said plasma separating section so that a blood sample is introduced into said plasma separating section when the centrifugal force is applied to the substrate in the first centrifugal direction;

(d) a calibrator solution introducing channel for communicating with said plasma separating section so that a calibrator solution is introduced into said plasma separating section, when the centrifugal force is applied to the substrate in the first centrifugal direction;

(e) a calibrator solution waste reservoir for communicating with said plasma separating section and allowing the calibrator solution in said plasma separating section to move therein, when the centrifugal force is applied to the substrate in a second centrifugal direction; and

(f) a calibrator solution discharge channel which allows said plasma separating section to communicate with said calibrator solution waste reservoir, and discharges the calibrator solution in said plasma separating section to said calibrator solution waste reservoir, when the centrifugal force is applied to the substrate in the second centrifugal direction;

wherein said sensor groove is positioned to discharge the calibrator solution from the sensor groove by action of the centrifugal force in the second centrifugal direction; and

wherein said calibrator solution waste reservoir is positioned and said calibrator solution discharge channel is disposed so that the calibrator solution contained in the calibrator solution waste reservoir does not flow back into said plasma separating section when the centrifugal force is applied to the substrate in the first centrifugal direction.