| US 7,416,893 B2 | ||
| Methods for diagnosing drug-resistant cancer cells or for identifying chemotherapeutic agents by measuring anomalous intracellular ion and/or second messenger dynamics | ||
| Maria A. DeBernardi, Potomac, Md. (US); and Gary Brooker, Rockville, Md. (US) | ||
| Assigned to Atto Bioscience, Rockville, Md. (US) | ||
| Filed on Dec. 06, 2002, as Appl. No. 10/313,039. | ||
| Prior Publication US 2004/0110244 A1, Jun. 10, 2004 | ||
| Int. Cl. G01N 33/48 (2006.01); G01N 33/483 (2006.01) | ||
| U.S. Cl. 436—34 [436/64; 436/79] | 25 Claims |
| 1. A method for determining the resistance of a cell to a drug comprising:
(a) determining an intracellular decay time for calcium ions in one or more test cell(s) and one or more control cell(s) after
exposure to an ion elevating agent, wherein the test cell(s) is a breast cancer cell or a glioblastoma cell,
(b) comparing the intracellular decay time for calcium ions in the test cell(s) with the intracellular decay time for calcium
ions in the control cell(s), and
(c) correlating a difference between the intracellular decay time for calcium ions in the test cell(s) and the intracellular
decay time for calcium ions in the control cell(s) to determine the resistance to the drug of the one or more test cell(s).
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