| US 7,579,195 B2 | ||
| Simple membrane assay method and kit | ||
| Hideharu Shimizu, Gosen (Japan); Takeshi Watanabe, Gosen (Japan); Kazuyuki Takizawa, Gosen (Japan); Junji Matsuda, Gosen (Japan); and Toshinori Sato, Gosen (Japan) | ||
| Assigned to Denka Seiken Co., Ltd, Tokyo (Japan) | ||
| Filed on Jun. 21, 2006, as Appl. No. 11/471,622. | ||
| Application 11/471622 is a continuation of application No. PCT/JP03/16615, filed on Dec. 24, 2003. | ||
| Prior Publication US 2007/0202611 A1, Aug. 30, 2007 | ||
| Int. Cl. G01N 33/53 (2006.01) | ||
| U.S. Cl. 436—518 [435/7.1; 435/805; 435/810; 436/514; 436/523; 436/531; 436/535; 436/807; 436/808; 436/810] | 14 Claims |
| 1. A simple membrane assay method for detecting or quantitating an analyte in a specimen sample using an assay device equipped
with a membrane bound with a capture-substance to capture the analyte and a filter tube consisting of a body part and a tip
part, comprising the steps of:
filtering the specimen sample using a filter attached to the tip part of the filter tube by applying pressure in the filter
tube,
dropping the filtrate onto said membranes, and
detecting or quantitating the presence of the analyte in said specimen sample,
wherein the method is a lateral-flow type membrane assay method, and
wherein the core size or the particle retention size of the membrane is not less than the pore size or the particle retention
size of the filter, the pore size or the particle retention size of said filter is from 0.2 to 1.0 μm, and the material of
said membrane is nitrocellulose and its pore size is from 0.4 to 12 μm.
|