| US 7,579,166 B2 | ||
| Glycoprotein and process for producing the same | ||
| Yasunori Chiba, Ibaraki (Japan); Yoshifumi Jigami, Ibaraki (Japan); Hitoshi Sakuraba, Tokyo (Japan); Kazuo Kobayashi, Gumma (Japan); and Makoto Takeuchi, Kanagawa (Japan); Yoriko Takeuchi, legal representative | ||
| Assigned to National Institute of Advanced Industrial Science and Technology, Tokyo (Japan); and Tokyo Metropolitan Organization for Medical Research, Tokyo (Japan) | ||
| Appl. No. 10/480,790 PCT Filed Jun. 14, 2002, PCT No. PCT/JP02/05965 § 371(c)(1), (2), (4) Date Jun. 24, 2004, PCT Pub. No. WO02/103027, PCT Pub. Date Dec. 27, 2002. |
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| Claims priority of application No. 2001-180907 (JP), filed on Jun. 14, 2001. | ||
| Prior Publication US 2005/0064539 A1, Mar. 24, 2005 | ||
| Int. Cl. C12N 15/00 (2006.01); C12P 21/00 (2006.01); C12Q 1/68 (2006.01); C07K 14/00 (2006.01) | ||
| U.S. Cl. 435—69.1 [435/6; 435/71.1] | 11 Claims |
| 1. A process for producing an active form of a glycoprotein comprising an acidic sugar chain having a mannose-6-phosphate
at a non-reducing end, comprising
(i) expressing a gene introduced into yeast encoding said glycoprotein; and
(ii) treating said glycoprotein with α-mannosidase obtained from Cellulomonas SO-5 strain bacteria (FERM BP-7628) to remove a mannose residue from the mannose- 1-phosphate in the sugar chain of the glycoprotein,
wherein a-1,6-mannosyltransferase gene and a-1,3-mannosyltransferase gene have been disrupted in said yeast.
|