| US 7,579,151 B2 | ||
| Isolation and purification method of biomolecules using hydrogel | ||
| Young-sun Lee, Gyeonggi-do (Korea, Republic of); Jung-im Han, Seoul (Korea, Republic of); Hyo-yeon Lee, Gyeonggi-do (Korea, Republic of); Kak Namkoong, Seoul (Korea, Republic of); and Kwang-wook Oh, Gyeonggi-do (Korea, Republic of) | ||
| Assigned to Samsung Electronics Co., Ltd., (Korea, Republic of) | ||
| Filed on Jul. 27, 2005, as Appl. No. 11/191,221. | ||
| Claims priority of application No. 10-2004-0104025 (KR), filed on Dec. 10, 2004. | ||
| Prior Publication US 2006/0127887 A1, Jun. 15, 2006 | ||
| Int. Cl. C12Q 1/68 (2006.01); C07H 21/00 (2006.01) | ||
| U.S. Cl. 435—6 [536/25.4] | 15 Claims |
| 1. A method of isolating and purifying nucleic acid using an anionic hydrogel, the method comprising:
bringing a sample containing charged nucleic acid into contact with an anionic hydrogel to bind the nucleic acid to the anionic
hydrogel,
wherein the anionic hydrogel consists of a polymerizable acid or a polymerizable acid salt, and is swollen by water or electrolysis;
washing the anionic hydrogel bound with the nucleic acid; and
eluting the bound nucleic acid using an elution solvent,
wherein the method is conducted in the absence of magnetic beads.
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