| US 7,413,895 B2 | ||
| DNA encoding neutral amino acid transporter | ||
| Hitoshi Endou, Sagamihara (Japan); and Yoshikatsu Kanai, Hachioji (Japan) | ||
| Assigned to Japan Science and Technology Corporation, Saitama (Japan) | ||
| Filed on Aug. 05, 2004, as Appl. No. 10/913,256. | ||
| Application 10/913256 is a division of application No. 09/786389, previously published as PCT/JP99/04789, filed on Sep. 03, 1999. | ||
| Claims priority of application No. 10-249993 (JP), filed on Sep. 03, 1998; and application No. 11-248546 (JP), filed on Sep. 02, 1999. | ||
| Prior Publication US 2005/0019811 A1, Jan. 27, 2005 | ||
| Int. Cl. C12N 15/12 (2006.01); C12N 15/63 (2006.01); C12N 5/10 (2006.01) | ||
| U.S. Cl. 435—320.1 [435/325; 536/23.5] | 15 Claims |
| 1. An isolated DNA encoding a protein which is a cell surface protein having an ability to mediate the transport of an amino
acid into a cell, the cell surface protein further having an ability to mediate incorporation of at least one amino acid selected
from the group consisting of leucine (Leu), isoleucine (Ile), phenylalanine (Phe), methionine (Met), tyrosine (Tyr), tryptophan
(Trp), valine (Val), histidine (His) and other neutral amino acids into the cell in an Na+-independent manner, wherein the protein has an amino acid sequence selected from a) to d):
a) amino acid sequence represented by SEQ ID NO:2,
b) amino acid sequence represented by SEQ ID NO:4,
c) amino acid sequence represented by SEQ ID NO:2, wherein 1 to 20 amino acid residue(s) is (are) substituted, deleted or
added,
d) amino acid sequence represented by SEQ ID NO:4, wherein 1 to 20 amino acid residues) is (are) substituted, deleted or added.
|