	US 7,576,192 B2
	Rapid and sensitive assay for the detection and quantification of coregulators of nucleic acid binding factors
Tomasz Heyduk, Ballwin, Mo. (US)
Assigned to Saint Louis University, St. Louis, Mo. (US)
Filed on Dec. 12, 2006, as Appl. No. 11/609,628.
Application 11/609628 is a division of application No. 10/062064, filed on Jan. 31, 2002, granted, now 7,172,865.
Application 10/062064 is a continuation in part of application No. 09/928385, filed on Aug. 13, 2001, granted, now 6,544,746, filed on Apr. 08, 2003.
Prior Publication US 2008/0044826 A1, Feb. 21, 2008
Int. Cl. C07H 21/04 (2006.01); C12N 15/11 (2006.01)

U.S. Cl. 536—23.1 [435/6; 977/792]

5 Claims

1. An array of biosensors useful for determining the activity of nucleic acid binding protein coregulators comprising at least two biosensors, wherein each of said biosensors comprises a first nucleic acid component, a second nucleic acid component and a nucleic acid binding factor, wherein

a. the first nucleic acid component comprises a portion of a nucleic acid binding element and the second nucleic acid component comprises a portion of the same nucleic acid binding element wherein the combination of both the first and second nucleic acid components comprises the nucleic acid binding element; and

b. the first nucleic acid component is attached to a solid substrate and the second nucleic acid is attached to a detectable label, wherein the label is detected upon binding of the nucleic acid binding factor to the first and second nucleic acid components, to form the nucleic acid binding element, said binding being enhanced or disrupted by the nucleic acid binding protein coregulator.