US 7,576,070 B2
Method for producing pectin hydrolysis products
Markwart Kunz, Worms (Germany); Mohammad Munir, Kindenheim (Germany); and Manfred Vogel, Neuleiningen (Germany)
Assigned to N.V. Nutricia, (Netherlands)
Appl. No. 10/416,347
PCT Filed Nov. 21, 2001, PCT No. PCT/EP01/13508
§ 371(c)(1), (2), (4) Date Sep. 29, 2003,
PCT Pub. No. WO02/42484, PCT Pub. Date May 30, 2002.
Claims priority of application No. 100 57 976 (DE), filed on Nov. 22, 2000.
Prior Publication US 2004/0072791 A1, Apr. 15, 2004
Int. Cl. A61K 31/732 (2006.01); C12P 19/04 (2006.01)
U.S. Cl. 514—54  [435/18; 435/19; 435/72; 435/101; 435/201; 435/232] 8 Claims
 
1. Method for producing pectin hydrolysis products, wherein a pectin or pectin-containing plant material in aqueous solution or suspension is treated in a first step with a pectin-hydrolyzing enzyme A and in a second step with a pectin-hydrolyzing enzyme B, and after enzyme treatments, recovering pectin hydrolysis products with a content of galacturonides that include at least one 4,5-unsaturated galacturonic acid molecule and are esterified >30% with methanol, wherein enzyme A is a pectinlyase (EC 4.2.2.10) and enzyme B is an endopolygalacturonase (EC 3.2.1.15).