US 7,575,875 B2
	Method of tacrolimus extraction and quantification using aqueous detergents
John G. Konrath, Lake Villa, Ill. (US); and David H. Wilson, Zion, Ill. (US)
Assigned to Abbott Laboratories, Inc., Abbott Park, Ill. (US)
Filed on Oct. 13, 2005, as Appl. No. 11/249,188.
Prior Publication US 2007/0087396 A1, Apr. 19, 2007
Int. Cl. G01N 33/00 (2006.01); A01N 1/02 (2006.01)

U.S. Cl. 435—7.1  [435/2; 436/501; 436/518; 436/522; 422/50; 422/61]

6 Claims

1. A method of extracting tacrolimus from a blood sample, comprising contacting the blood sample with a non-ionic detergent hemolytic agent that lyses the cells of said sample and releases tacrolimus protein complexes and a water soluble detergent extraction agent that dissociates tacrolimus from the tacrolimus protein complexes, wherein said hemolytic agent is octylphenoxy polyethoxyethanol and said extraction agent is deoxycholate and wherein said hemolytic agent and said extraction agent do not include sodium dodecyl sulfate, and contacting is done such that lysis and dissociation takes place and tacrolimus is extracted from said sample.