| US 7,575,856 B2 | ||
| Compositions and methods for the evaluation and resuscitation of cadaveric hearts for transplant | ||
| Shukri Khuri, Westwood, Mass. (US); Hemant Thatte, Medfield, Mass. (US); and Vladimir Birjinuik, Weston, Mass. (US) | ||
| Assigned to The United States of America as represented by the Department of Veterans Affairs, Washington, D.C. (US); and President and Fellows of Harvard College, Cambridge, Mass. (US) | ||
| Filed on Oct. 28, 2005, as Appl. No. 11/261,860. | ||
| Prior Publication US 2007/0098694 A1, May 03, 2007 | ||
| Int. Cl. A01N 1/02 (2006.01) | ||
| U.S. Cl. 435—1.2 [435/1.1; 436/63; 436/148; 436/163; 514/2; 514/18; 514/474] | 1 Claim |
| 1. A method of treating a candidate cadaveric heart comprising:
a) providing an arrested candidate cadaveric heart;
b) perfusing said candidate cadaveric heart with a physiological solution, wherein said physiological solution consists of
Hank's balanced salt solution, ascorbic acid, reduced glutathione, L-arginine, heparin, adenosine, guanosine, inosine, ribose,
lipids, red blood cells, albumin, insulin, epinephrine and aspirin;
c) converting said perfused arrested candidate cadaveric heart into a beating heart;
d) measuring the pH of the anterior aspect of the myocardium of said beating candidate cadaveric heart, and
e) transplanting said cadaveric heart when the pH, as measured in step d), is greater than 6.8 and a left ventricular end
diastolic pressure is less than 30 mmHg.
|