| US 7,410,637 B2 | ||
| Transgenic algae for delivering antigens to an animal | ||
| Richard T. Sayre, Worthington, Ohio (US); Richard E. Wagner, Bloomington, Ind. (US); Surasak Siripornadulsil, Columbus, Ohio (US); and Carlos Farias, Valdivia (Chile) | ||
| Assigned to Phycotransgenics, LLC, Bloomington, Ind. (US); and The Ohio State University, Columbus, Ohio (US) | ||
| Appl. No. 10/311,741 PCT Filed Jun. 20, 2001, PCT No. PCT/US01/19643 § 371(c)(1), (2), (4) Date Dec. 18, 2002, PCT Pub. No. WO01/98335, PCT Pub. Date Dec. 27, 2001. |
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| Claims priority of provisional application 60/212757, filed on Jun. 20, 2000. | ||
| Prior Publication US 2003/0211089 A1, Nov. 13, 2003 | ||
| Int. Cl. A01N 63/00 (2006.01); A61K 39/00 (2006.01); C12P 21/06 (2006.01); C12N 5/00 (2006.01); C12N 15/00 (2006.01); C12N 15/82 (2006.01) | ||
| U.S. Cl. 424—93.2 [424/93.1; 424/184.1; 435/69.1; 435/320.1; 435/410; 435/468] | 32 Claims |
| 1. A delivery system for delivering a biologically active protein to a host animal comprising
an algal cell transformed by an expression vector, the expression vector comprising
a β-tubulin promoter of Chlamydomonas,
a 3′-terminator from the Chlamydomonas low C2-induced membrane protein gene having the sequence of SEQ ID NO. 4, and
a nucleotide sequence coding for the biologically active protein fused to an end of the low CO2-induced plasma membrane protein gene SEQ ID NO. 3, said fused nucleotide sequence positioned between the β-tubulin promoter
and the 3′ terminator and operably linked to the β-tubulin promoter.
|