CPC C12Q 1/6881 (2013.01) [C12Q 1/6883 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/158 (2013.01)] | 8 Claims |
1. A kit for use in carrying out a method for detecting microchimerism, the kit comprising one or more specific nucleic acid molecule compositions comprising a forward nucleic acid molecule comprising the sequence set forth in SEQ ID NO: 64, a reverse nucleic acid molecule comprising the sequence set forth in SEQ ID NO: 65, and a probe nucleic acid molecule comprising the sequence set forth in SEQ ID NO: 66;
wherein the method comprises:
(a) amplifying nucleic acid molecules of a target biological sample using the specific nucleic acid molecule compositions;
(b) amplifying nucleic acid molecules of a control biological sample using the specific nucleic acid molecule compositions of step (a);
(c) comparing the amount of amplified nucleic acid molecules with a reference to quantitate specific nucleic acid molecule markers in the samples;
wherein each specific nucleic acid molecule composition amplifies a SE-LR genetic polymorphism; and
wherein the presence of certain specific amplified markers in the target biological sample indicates the presence of microchimerism.
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