US 11,718,875 B2
Compositions and methods for detecting allogeneic matter in a subject
J. Lee Nelson, Seattle, WA (US); Nathalie C. Lambert, Marseilles (FR); Vijayakrishna K. Gadi, Seattle, WA (US); and Zhen Yan, Irving, TX (US)
Assigned to Fred Hutchinson Cancer Center, Seattle, WA (US)
Filed by Fred Hutchinson Cancer Center, Seattle, WA (US)
Filed on Feb. 19, 2020, as Appl. No. 16/795,375.
Application 16/795,375 is a division of application No. 14/382,105, granted, now 10,604,805, previously published as PCT/US2013/028733, filed on Mar. 1, 2013.
Claims priority of provisional application 61/605,680, filed on Mar. 1, 2012.
Prior Publication US 2020/0181702 A1, Jun. 11, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6881 (2018.01); C12Q 1/6883 (2018.01)
CPC C12Q 1/6881 (2013.01) [C12Q 1/6883 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/158 (2013.01)] 8 Claims
OG exemplary drawing
 
1. A kit for use in carrying out a method for detecting microchimerism, the kit comprising one or more specific nucleic acid molecule compositions comprising a forward nucleic acid molecule comprising the sequence set forth in SEQ ID NO: 64, a reverse nucleic acid molecule comprising the sequence set forth in SEQ ID NO: 65, and a probe nucleic acid molecule comprising the sequence set forth in SEQ ID NO: 66;
wherein the method comprises:
(a) amplifying nucleic acid molecules of a target biological sample using the specific nucleic acid molecule compositions;
(b) amplifying nucleic acid molecules of a control biological sample using the specific nucleic acid molecule compositions of step (a);
(c) comparing the amount of amplified nucleic acid molecules with a reference to quantitate specific nucleic acid molecule markers in the samples;
wherein each specific nucleic acid molecule composition amplifies a SE-LR genetic polymorphism; and
wherein the presence of certain specific amplified markers in the target biological sample indicates the presence of microchimerism.