	US 11,718,857 B2
	Broad host range genetic tools for engineering microalgae
Michael T. Guarnieri, Denver, CO (US); Jeffrey George Linger, Denver, CO (US); and Lukas Royce Dahlin, Golden, CO (US)
Assigned to Alliance for Sustainable Energy, LLC, Golden, CO (US)
Filed by Alliance for Sustainable Energy, LLC, Golden, CO (US)
Filed on Aug. 10, 2020, as Appl. No. 16/989,549.
Claims priority of provisional application 62/884,918, filed on Aug. 9, 2019.
Prior Publication US 2021/0071187 A1, Mar. 11, 2021
Int. Cl. C12N 15/82 (2006.01); C12N 1/12 (2006.01)

CPC C12N 15/8209 (2013.01) [C12N 1/12 (2013.01)]

8 Claims

1. A method for transforming a Picochlorum renovo algal chloroplast, the method comprising:

introducing an exogenous selection marker into the Picochlorum renovo algal chloroplast; and

bombarding a culture of the Picochlorum renovo algal chloroplast with a loaded particle.