CPC C12N 9/2417 (2013.01) [C12N 9/2414 (2013.01); C12Q 1/40 (2013.01)] | 14 Claims |
1. A method for screening variant alpha-amylases of a parent alpha-amylase of SEQ ID NO: 2, comprising the steps of
a) determining the binding of the alpha-amylases to a solid or an immobilized substrate for the alpha amylase; and
b) selecting variant alpha-amylases having a lower binding to solid starch and higher wash performance at low temperature compared with the parent alpha-amylase, wherein the variant alpha-amylases have at least 80% sequence identity but less than 100% sequence identity to SEQ ID NO: 2 and comprise a deletion of positions 181+182, 182+183, or 183+184, and further comprise one or two or more substitutions in any of positions corresponding to W159, W167, Q169, W189, E194, N260, F262, W284, F289, G304, G305, W347, W469, G476 and G477 in SEQ ID NO: 2.
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