CPC C12N 9/1252 (2013.01) [C12Y 207/07007 (2013.01)] | 5 Claims |
1. A DNA polymerase comprising an amino acid sequence having at least 80% sequence identity to SEQ ID NO: 1, wherein the DNA polymerase:
(A) has a lower 3′→5′ exonuclease activity than a reference DNA polymerase, wherein the reference DNA polymerase consists of SEQ ID NO: 1,
(B) has the same or higher strand displacement activity than the reference DNA polymerase, and
(C) has a set of substitutions relative to SEQ ID NO: 1, and wherein at least one substitution of the set of substitutions is at a position selected from the group consisting of L125K, D128H, V179Y, V179R, S211F, Y212K, I215L, T216K, Q221R, Y242G/A/L/S, Y259G/K/Q, A292K, S293G, and S294T.
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