US 11,718,836 B2
Exonuclease deficient polymerases
Aruna Ayer, Santa Clara, CA (US); Arkadiusz Bibillo, Cupertino, CA (US); Preethi Sarvabhowman, Santa Clara, CA (US); Dhruti Vasudev Dalal, Sunnyvale, CA (US); Ilya Lederman, San Francisco, CA (US); and Eileen Thai, San Jose, CA (US)
Assigned to Roche Sequencing Solutions, Inc., Pleasanton, CA (US)
Filed by Roche Sequencing Solutions, Inc., Pleasanton, CA (US)
Filed on Feb. 5, 2021, as Appl. No. 17/248,768.
Application 17/248,768 is a division of application No. 16/280,890, filed on Feb. 20, 2019, granted, now 10,947,516.
Application 16/280,890 is a division of application No. 15/444,020, filed on Feb. 27, 2017, granted, now 10,266,813, issued on Apr. 23, 2019.
Claims priority of provisional application 62/301,475, filed on Feb. 29, 2016.
Prior Publication US 2021/0163904 A1, Jun. 3, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/12 (2006.01)
CPC C12N 9/1252 (2013.01) [C12Y 207/07007 (2013.01)] 5 Claims
 
1. A DNA polymerase comprising an amino acid sequence having at least 80% sequence identity to SEQ ID NO: 1, wherein the DNA polymerase:
(A) has a lower 3′→5′ exonuclease activity than a reference DNA polymerase, wherein the reference DNA polymerase consists of SEQ ID NO: 1,
(B) has the same or higher strand displacement activity than the reference DNA polymerase, and
(C) has a set of substitutions relative to SEQ ID NO: 1, and wherein at least one substitution of the set of substitutions is at a position selected from the group consisting of L125K, D128H, V179Y, V179R, S211F, Y212K, I215L, T216K, Q221R, Y242G/A/L/S, Y259G/K/Q, A292K, S293G, and S294T.