CPC C12N 5/0068 (2013.01) [B05D 3/0254 (2013.01); C12M 23/20 (2013.01); C12N 5/0037 (2013.01); C12N 5/0056 (2013.01); C12N 5/0606 (2013.01); C12N 5/0663 (2013.01); C12N 2500/90 (2013.01); C12N 2500/98 (2013.01); C12N 2533/30 (2013.01); C12N 2533/40 (2013.01); C12N 2533/50 (2013.01)] | 20 Claims |
1. A method for coating a surface of a cell culture article, comprising:
(i) introducing a polypeptide-polymer comprising a polymer having a covalently attached polypeptide to an aqueous cell culture medium to produce a polymer containing cell culture medium, wherein the polymer is selected from the group consisting of a polymer that has a linear backbone and is crosslink free, a polymer that has a branched backbone and that is crosslink free, and a star polymer that is crosslink free, wherein the polypeptide-polymer is soluble at 20° C. or less, wherein the aqueous solution is substantially free of organic solvents, and wherein the molar ratio of the polypeptide-polymer to polymer not covalently attached to polypeptide in the aqueous solution is from about 1:1 to about 1:50;
(ii) disposing the polymer containing cell culture medium on the surface of the cell culture article to produce a coated article; and
(iii) incubating the coated article in the medium under cell culture conditions to attach the polymer conjugated to the polypeptide to the surface of the cell culture article.
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