CPC C07K 14/55 (2013.01) [A61P 35/00 (2018.01); C07K 14/5443 (2013.01); A61K 38/00 (2013.01); C07K 2317/52 (2013.01); C07K 2317/94 (2013.01); C07K 2319/30 (2013.01)] | 9 Claims |
1. A masked cytokine comprising
a first polypeptide H-linker-C and
a second polypeptide H-linker-MM,
wherein H is a Fc domain, C is an IL-12 cytokine, and MM comprises an extracellular domain of IL-12Rβ2;
wherein the linker in the first polypeptide or second polypeptide is cleavable; and
wherein the Fc domain in the second polypeptide comprises Y349C, T366S, L368A and Y407 mutations and the Fc domain in the first polypeptide comprises S354C and T366W mutations relative to SEQ ID NO: 154 by EU numbering.
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