US 11,718,655 B2
Masked interleukin-12 polypeptides
Margaret Karow, Santa Rosa Valley, CA (US); Deborah Moore Lai, Beverly, MA (US); Dheeraj Tomar, Dorchester, MA (US); Parker Johnson, Allston, MA (US); Raphael Rozenfeld, Newton, MA (US); Ronan O'Hagan, Waltham, MA (US); and Huawei Qiu, Westborough, MA (US)
Assigned to XILIO DEVELOPMENT, INC., Waltham, MA (US)
Filed by Xilio Development, Inc., Waltham, MA (US)
Filed on Apr. 8, 2022, as Appl. No. 17/716,873.
Application 17/716,873 is a continuation of application No. 17/279,407, previously published as PCT/US2019/053588, filed on Sep. 27, 2019.
Claims priority of provisional application 62/737,803, filed on Sep. 27, 2018.
Claims priority of provisional application 62/888,276, filed on Aug. 16, 2019.
Claims priority of provisional application 62/891,199, filed on Aug. 23, 2019.
Prior Publication US 2022/0306716 A1, Sep. 29, 2022
Int. Cl. A61P 35/00 (2006.01); A61K 38/00 (2006.01); C07K 14/55 (2006.01); C07K 14/54 (2006.01)
CPC C07K 14/55 (2013.01) [A61P 35/00 (2018.01); C07K 14/5443 (2013.01); A61K 38/00 (2013.01); C07K 2317/52 (2013.01); C07K 2317/94 (2013.01); C07K 2319/30 (2013.01)] 9 Claims
 
1. A masked cytokine comprising
a first polypeptide H-linker-C and
a second polypeptide H-linker-MM,
wherein H is a Fc domain, C is an IL-12 cytokine, and MM comprises an extracellular domain of IL-12Rβ2;
wherein the linker in the first polypeptide or second polypeptide is cleavable; and
wherein the Fc domain in the second polypeptide comprises Y349C, T366S, L368A and Y407 mutations and the Fc domain in the first polypeptide comprises S354C and T366W mutations relative to SEQ ID NO: 154 by EU numbering.