CPC A23K 10/12 (2016.05) [A23K 10/14 (2016.05); A23K 10/18 (2016.05); A23K 10/37 (2016.05); A23K 20/189 (2016.05); A23K 40/25 (2016.05); C12M 29/18 (2013.01); C12M 43/00 (2013.01); C12M 45/00 (2013.01); C12M 47/10 (2013.01); C12M 47/12 (2013.01); C12P 1/04 (2013.01)] | 15 Claims |
1. A method of processing meal obtained from oilseed after oil extraction using biotransformation, the method comprising the steps of:
pretreating oilseed obtained meal in a meal tank;
mixing said oilseed obtained meal after the step of pretreating, additives stimulating biosynthesis of products, water from a water tank, and microorganisms to water content of 20% to 85% in a bioreactor, said microorganisms being bacteria;
incubating in said bioreactor from 6 to 48 hours at 20 to 50 degrees Celsius so as to ferment and form a mixture being comprised of a liquid fraction and a solid fraction;
directing the mixture to a separator;
separating said solid fraction with said separator;
drying said solid fraction at a temperature not exceeding denaturalization temperature of enzymes with a bio-meal dryer so as to form a dried solid fraction;
dispensing said dried solid fraction into a supplementary feed mix component tank;
separating said liquid fraction with said separator;
centrifuging said liquid fraction with a centrifuge so as to separate microorganism cells and solid impurities of diameter sizes greater than 0.1 μm from a purified liquid fraction in said liquid fraction;
directing said microorganism cells and solid impurities of diameter sizes greater than 0.1 μm back to said bioreactor;
directing said purified liquid fraction for further separation processing,
wherein further separation processing is comprised of at least one of a first option, a second option, a third option, and a fourth option,
wherein said first option is comprised of the steps of:
directing said purified liquid fraction to an absorber;
carrying out sorption of bio surfactants produced by microorganisms on activated carbon so as to generate adsorbed bio surfactants;
drying in a dryer, after the step of carrying out sorption; and
carrying out desorption of said adsorbed bio surfactants by CO2 extraction under supercritical conditions of activated carbon with said adsorbed bio surfactants, in a pressure reactor, so as to generate separated raw surfactant solid fraction and a surfactant separated liquid fraction,
wherein said second option is comprised of the steps of:
directing said purified liquid fraction to an absorber;
carrying out sorption of bio surfactants produced by microorganisms on activated carbon; and
extracting in a reactor at atmospheric pressure so as to generate said separated raw surfactant solid fraction and said surfactant separated liquid fraction,
wherein said third option is comprised of the steps of:
directing said purified liquid fraction to another reactor; and
precipitating said separated raw surfactant solid fraction in said surfactant separated liquid fraction, and
wherein said fourth option is comprised of the steps of:
directing said purified liquid fraction to a dehydrator;
dehydrating so as to generate said separated raw surfactant solid fraction and said surfactant separated liquid fraction; and
directing an aqueous solution to said water tank;
directing said separated raw surfactant solid fraction to a raw surfactant tank;
directing said surfactant separated liquid fraction to a condenser, said surfactant separated liquid fraction containing polymers;
concentrating said surfactant separated liquid fraction so as to generate a concentrated liquid fraction;
directing said concentrated liquid fraction from said condenser to a polymer separator;
separating polymers from said concentrated liquid fraction with ethanol in said polymer separator so as to generate a raw polymer form and a raw polymer separated liquid fraction;
directing said raw polymer form to a raw polymer tank;
dispensing said raw polymer separated liquid fraction dispensed to an ethanol recovery device;
separating ethanol from said raw polymer separated liquid fraction with said ethanol recovery device so as to generate separated ethanol and ethanol pure liquid fraction containing bioproducts;
directing said separated ethanol to said polymer separator;
directing said ethanol pure liquid fraction to said bioreactor;
directing said raw surfactants from said raw surfactant tank to purified surfactin tanks so as to refine said raw surfactants; and
directing said raw polymer form from said raw polymer tank to purified polymer tanks in a polymer fractionation device and/or surfactant fractionation device.
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