| US 7,572,607 B2 | ||
| Polypeptides and biosynthetic pathways for the production of monatin and its precursors | ||
| Paula M. Hicks, Bend, Oreg. (US); Sara C. McFarlan, St. Paul, Minn. (US); Timothy W. Abraham, Minnetonka, Minn. (US); Douglas C. Cameron, Plymouth, Minn. (US); James R. Millis, Plymouth, Minn. (US); John Rosazza, Iowa City, Iowa (US); Lishan Zhao, Carlsbad, Calif. (US); and David Paul Weiner, Del Mar, Calif. (US) | ||
| Assigned to Cargill, Incorporated, Wayzata, Minn. (US) | ||
| Filed on Apr. 26, 2005, as Appl. No. 11/114,922. | ||
| Application 11/114922 is a continuation in part of application No. 10/422366, filed on Apr. 23, 2003, abandoned. | ||
| Claims priority of provisional application 60/374831, filed on Apr. 23, 2002. | ||
| Prior Publication US 2005/0282260 A1, Dec. 22, 2005 | ||
| Int. Cl. C12P 13/22 (2006.01); C12N 1/21 (2006.01) | ||
| U.S. Cl. 435—121 [435/108; 435/193; 435/69.1; 435/325; 435/320.1; 435/252.3; 536/23.2] | 45 Claims |
| 1. A method for producing 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid comprising
contacting indole-3-pyruvate with a polypeptide chosen from KHG aldolase (4-hydroxy-2-oxoglutarate glyoxylate-lyase, EC 4.1.3.16)
and ProA aldolase (4-hydroxy-4-methyl-2-oxoglutarate pyruvate-lyase, EC 4.1.3.17) under conditions in which 2-hydroxy 2-(indol-3-ylmethyl)-4-keto
glutaric acid is produced.
|