US 7,407,747 B2
	Method for drying dye-terminator sequencing reagents
Kathleen M. Perry, San Francisco, Calif. (US); Robert E. Ruhfel, San Francisco, Calif. (US); Sandra L. Spurgeon, San Mateo, Calif. (US); and Peter Ma, Cupertino, Calif. (US)
Assigned to Applera Corporation, Foster City, Calif. (US)
Filed on Oct. 15, 2002, as Appl. No. 10/272,450.
Prior Publication US 2004/0072167 A1, Apr. 15, 2004
Int. Cl. C12Q 1/68 (2006.01); C07H 21/00 (2006.01); C07H 21/02 (2006.01); C12P 19/34 (2006.01)

U.S. Cl. 435—6  [435/91.1; 435/91.2; 536/23.1; 536/25.3; 536/26.6]

15 Claims

1. A dry composition comprising a mononucleotide linked to a fluorescent dye, wherein the composition comprises a thermostable nucleic acid polymerase, wherein the thermostable nucleic acid polymerase is Taq DNA polymerase, wherein the dry composition is substantially free of glycerol, and wherein the ratio of glycerol to Taq DNA polymerase is less than 1 ug of glycerol per unit of Taq DNA polymerase.