| US 7,569,398 B2 | ||
| Methods and devices for transporting and concentrating an analyte present in a sample | ||
| Pierre Puget, Saint Ismier (France); Patrick Pouteau, Voreppe (France); Frédéric Ginot, St Egreve (France); and Patrice Caillat, Echirolles (France) | ||
| Assigned to Commissariat a l'Energie Atomique, Paris (France) | ||
| Appl. No. 10/416,207 PCT Filed Nov. 27, 2001, PCT No. PCT/FR01/03743 § 371(c)(1), (2), (4) Date May 08, 2003, PCT Pub. No. WO02/43865, PCT Pub. Date Jun. 06, 2002. |
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| Claims priority of application No. 00 15417 (FR), filed on Nov. 29, 2000. | ||
| Prior Publication US 2004/0023273 A1, Feb. 05, 2004 | ||
| Int. Cl. G01N 33/543 (2006.01); G01N 33/553 (2006.01) | ||
| U.S. Cl. 436—518 [436/523; 436/524; 436/526; 436/534; 436/541; 436/540] | 10 Claims |
| 1. A method of concentrating an analyte present in a sample, comprising the following steps:
providing a solution A in which the analyte is attached to magnetic particles, by contacting the magnetic particles with the
sample, in a first container of volume α connected via a bottleneck to a second container of volume β, the volume β being
smaller than the volume α,
moving the analyte attached to the magnetic particles, by means of a magnetic system, from the first container to the bottleneck,
releasing the analyte attached to the magnetic particles from said particles in the bottleneck,
moving the magnetic particles released from the analyte from the bottleneck to said first container by means of a magnetic
system, and
transporting the analyte from the bottleneck to the second container by movement of the solution A.
|