| US 7,569,391 B2 | ||
| Method for making β-secretase co-crystals complexed with a ligand | ||
| Brian M. Beyer, Lincroft, N.J. (US); Bruce A. Malcolm, Paoli, Pa. (US); Corey O. Strickland, Martinsville, N.J. (US); Wenyan Wang, Edison, N.J. (US); and Eileen Wilson, Raritan, N.J. (US) | ||
| Assigned to Schering Corporation, Kenilworth, N.J. (US) | ||
| Filed on Dec. 06, 2006, as Appl. No. 11/634,737. | ||
| Application 11/634737 is a division of application No. 10/443949, filed on May 22, 2003, granted, now 7,166,454. | ||
| Claims priority of provisional application 60/383480, filed on May 24, 2002. | ||
| Prior Publication US 2007/0099273 A1, May 03, 2007 | ||
| Int. Cl. G01N 31/00 (2006.01); G01N 33/00 (2006.01) | ||
| U.S. Cl. 436—4 [435/183; 117/927] | 1 Claim |
1. A method for making a crystal comprising a polypeptide complexed to a
 (a) combining equal volumes of (i) a mixture of 16 mg/ml of polypeptides of SEQ ID NO: 20 and SEQ ID NO: 22 wherein said polypeptides
are generated by trans-cleavage processing of a polypeptide of SEQ ID NO: 18; and 1.0 mM of OM-99-2 ligand; with (ii) a crystallization
solution, said solution consisting essentially of water, 20% PEG3350 and 0.2 M K+/Na+ tartrate; and
(b) crystallizing said combination at about 4° C. using a hanging drop method.
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