| US 7,569,366 B2 | ||
| PCR amplification reaction apparatus and method for PCR amplification reaction using apparatus | ||
| Mamoru Tsukada, Kanagawa-ken (Japan) | ||
| Assigned to Canon Kabushiki Kaisha, Tokyo (Japan) | ||
| Filed on Apr. 22, 2005, as Appl. No. 11/111,904. | ||
| Claims priority of application No. 2004-130041 (JP), filed on Apr. 26, 2004. | ||
| Prior Publication US 2005/0239119 A1, Oct. 27, 2005 | ||
| Int. Cl. C12P 19/34 (2006.01); C12M 1/00 (2006.01) | ||
| U.S. Cl. 435—91.2 [435/6; 435/283.1] | 6 Claims |
| 1. A method for PCR amplification reaction comprising the steps of:
preparing a reaction vessel in which a heater is placed at a position to come into direct contact with a reaction solution
containing a nucleic acid chain;
preparing a heating block in which the reaction vessel is held;
maintaining the heating block at a temperature corresponding to an annealing phase;
setting the reaction solution into the reaction vessel; and
applying a row of pulsed current to the heater during a denaturing phase for producing pulsed heating corresponding to a pulse
time width and a pulse current height of the row of pulsed current to directly heat the reaction solution with the pulsed
heating,
wherein the heater is a coiled heater and is placed in the reaction vessel.
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