| US 7,569,341 B2 | ||
| Nucleic acid directed immobilization arrays and methods of assembly | ||
| Christof M. Niemeyer, Bremen (Germany); Charles R. Cantor, Del Mar, Calif. (US); Takeshi Sano, Boston, Mass. (US); and Cassandra L. Smith, Boston, Mass. (US) | ||
| Assigned to Trustees of Boston University, Boston, Mass. (US) | ||
| Filed on Nov. 07, 1997, as Appl. No. 8/967,269. | ||
| Application 08/967269 is a continuation of application No. 08/530912, filed on Sep. 20, 1995, abandoned. | ||
| Application 08/530912 is a continuation in part of application No. 08/189448, filed on Jan. 31, 1994, granted, now 5,561,043, filed on Oct. 01, 1996. | ||
| Prior Publication US 2003/0118595 A1, Jun. 26, 2003 | ||
| Int. Cl. C12Q 1/68 (2006.01) | ||
| U.S. Cl. 435—6 | 20 Claims |
| 1. A method for forming a nucleic acid-directed immobilization array of supramolecular bioconjugates comprising the steps
of:
a) attaching a first nucleic acid to a support in a regiospecific manner;
b) providing at least a second nucleic acid complementary to a sequence within said first nucleic acid, wherein said second
nucleic acid is coupled to a bioreactive agent, wherein said second bioreactive agent is a non-nucleic acid bioreactive agent
and contains a separate binding moiety that is selected to further react with a specific target;
c) providing at least a third nucleic acid complementary to a sequence within said first or second nucleic acid, wherein said
third nucleic acid is coupled to a bioreactive agent, wherein said third bioreactive agent is a non-nucleic acid bioreactive
agent and contains a separate binding moiety that is selected to further react with a specific target;
(d) hybridizing in one step said first nucleic acid to said second nucleic acid and said third nucleic acid to said first
or second nucleic acid to form a supramolecular bioconjugate; and
d) repeating steps (a)-(d) to form a plurality of immobilized supramolecular bioconjugates, wherein the separate binding moiety
of each of said bioreactive agent is selected to further react with a specific target.
|