US 7,569,184 B2
	Quantitative analysis of a biological sample of unknown quantity
Michael Wandell, Mercer Island, Wash. (US); Ilia Vinogradov, Elmhurst, Ill. (US); Barbara Godsey, Long Grove, Ill. (US); and Troy Bervig, Chicago, Ill. (US)
Assigned to Home Access Health Corporation, Hoffman Estates, Ill. (US)
Filed on Nov. 05, 2004, as Appl. No. 10/982,199.
Application 10/982199 is a continuation in part of application No. 10/706321, filed on Nov. 12, 2003.
Application 10/706321 is a continuation in part of application No. 10/421086, filed on Apr. 23, 2003.
Claims priority of provisional application 60/374629, filed on Apr. 23, 2002.
Prior Publication US 2005/0209532 A1, Sep. 22, 2005
Int. Cl. B01L 3/00 (2006.01); G01N 33/92 (2006.01); G01N 31/00 (2006.01); G01N 33/72 (2006.01)

U.S. Cl. 422—61  [436/71; 436/16; 436/17; 436/19; 436/66]

12 Claims

1. A method for determining the level of an analyte in blood from a solution formed from a dried blood fluid specimen, said blood fluid specimen being a plasma or serum specimen, comprising: in either order, measuring the analyte level in said solution and measuring the level of at least one normalizing analyte; and determining the analyte level in the blood from which said blood fluid specimen was collected based on said analyte level in said solution and on the level of said normalizing analyte in said solution, wherein, in determining the analyte level in the blood from which said blood fluid specimen was collected, a recovery delta is added to the analyte level of the analyte level in said solution to provide a recovery delta corrected solution analyte level, and the analyte level in the blood from which said blood fluid specimen was collected is determined as a function of said recovery delta corrected solution analyte level and on the level of said normalizing analyte, wherein said recovery delta is a positive value, wherein said recovery delta is determined as a function of the time elapsed since said blood fluid specimen was collected.