US 7,566,568 B2
	Method for generating highly active human dendritic cells from peripheral blood mononuclear cells
Filippo Belardelli, Rome (Italy); Stefano Maria Santini, Rome (Italy); Stefania Parlato, Rome (Italy); Tiziana Di Pucchio, Isola del Liri (Italy); Mariantonia Logozzi, Rome (Italy); Caterina Lapenta, Florence (Italy); Maria Ferrantini, Rome (Italy); Laura Santodonato, Rome (Italy); and Giuseppina D'Agostino, Rome (Italy)
Assigned to Istituto Superiore di Sanita, Rome (Italy)
Filed on Apr. 27, 2001, as Appl. No. 9/845,042.
Prior Publication US 2003/0092177 A1, May 15, 2003
Int. Cl. C12N 5/00 (2006.01); C12N 5/02 (2006.01)

U.S. Cl. 435—377  [435/384; 435/385]

22 Claims

1. A process for deriving dendritic cells from mononuclear cells in culture wherein said mononuclear cells are peripheral blood mononuclear cells (PBMC) or CD14+ monocytes, comprising culturing said mononuclear cells for a maximum of three days with type I interferon (IFN) at a concentration of 400 to 10,000 IU/ml in the presence of GM-CSF at a range of 250-1,000 IU/ml, and in the absence of IL-4, and recovering dendritic cells from said culture.