| US 7,566,547 B2 | ||
| Gene 763 of phytopathogenic fungus Magnaporthe grisea and use thereof for identifying fungicidal compounds | ||
| Marc-Henri LeBrun, Lyons (France); and Marie-Pascale Latorse, Sourcieux les Mines (France) | ||
| Assigned to Bayer CropScience S.A., (France) | ||
| Filed on Nov. 07, 2005, as Appl. No. 11/268,150. | ||
| Application 11/268150 is a division of application No. 10/240363, granted, now 7,070,981, previously published as PCT/FR01/00907, filed on Mar. 26, 2001. | ||
| Claims priority of application No. 00 04101 (FR), filed on Mar. 31, 2000. | ||
| Prior Publication US 2007/0122868 A1, May 31, 2007 | ||
| Int. Cl. A01N 25/00 (2006.01); A01N 65/00 (2006.01) | ||
| U.S. Cl. 435—7.31 [424/9.1; 424/404; 536/23.1; 435/471; 435/41] | 1 Claim |
| 1. A method for identifying compounds which inhibit fungal pathogenesis, comprising the following steps:
a) bringing a compound into contact with a host organism transformed with a polynucleotide comprising the promoter of gene
763 and a reporter gene, wherein the sequence of said promoter of gene comprises nucleotides 1-705 of SEQ ID NO: 1, and wherein
said host organism is a fungus, said host organism expressing said reporter gene under the control of said promoter of gene
763;
b) detecting the inhibition of the expression of said reporter gene, thereby identifying said compound as a candidate inhibitor
of fungal pathogenesis; and
c) determining whether the candidate compound identified in step (b) inhibits fungal pathogenesis, wherein inhibition of fungal
pathogenesis identifies the compound identified in step (b) as an inhibitor of fungal pathogenesis.
|