| US 7,566,530 B2 | ||
| Method for detecting PrP using at least one positive charge and/or at least one glycosidic bond and a ligand other than a protein ligand | ||
| Anna Benscik-Reynier, Saint Clair de la Tour (France); Anthony William Coleman, Caluire et Cuire (France); Eric Da Silva, Lyons (France); Marilyne Dupin, Vaugneray (France); Edwige Leclere, Lyons (France); Ambroise Martin, Charly (France); Aly Moussa, Oullins (France); Herve Perron, Saint Genis les Ollieres (France); and Frederic Ronzon, Montromant (France) | ||
| Assigned to Biomerieux, Marcy l'Etoile (France); Universite Claude Bernard Lyon, Villeubanne (France); Centre National de la Recherche Scientifique, Paris (France); and Agence Francaise de Securite Sanit Aire des Aliments, Maisons-Alfort (France) | ||
| Appl. No. 10/583,891 PCT Filed Jan. 19, 2005, PCT No. PCT/FR2005/000118 § 371(c)(1), (2), (4) Date Jun. 22, 2006, PCT Pub. No. WO2005/080590, PCT Pub. Date Sep. 01, 2005. |
||
| Claims priority of application No. 04 00492 (FR), filed on Jan. 20, 2004; and application No. 04 06538 (FR), filed on Jun. 17, 2004. | ||
| Prior Publication US 2008/0176256 A1, Jul. 24, 2008 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. G01N 33/53 (2006.01); C12Q 1/70 (2006.01); C12P 19/54 (2006.01); C12N 5/06 (2006.01) | ||
| U.S. Cl. 435—5 [435/7.1; 435/81; 435/337] | 12 Claims |
| 1. A method for detecting PrP in a biological sample of human or animal origin that may contain said PrP, comprising:
a) contacting the biological sample with a molecule selected from the group consisting of polyallylamine, triethylenetetraamine
(TET), bis-3-aminopropylamine, spermine tetrahydrochloride, dihydrostreptomycin sesquisulfate, streptomycin, and salts of
streptomycin to form PrP aggregates in a reaction mixture;
b) adding to the sample a macrocyclic ligand having general formula (I):
 where:
R1 represents a hydrogen atom, a hydroxyl group, an OR group or an OCOR group, R being as defined below,
R2 represents a hydrogen atom or an R, COR, Pol or CH2Pol group, in which Pol represents a phosphate, sulfate, amine, ammonium or carboxylic acid group, and R is as defined below,
R3 represents a hydrogen atom, a hydroxyl group, an OR group or an OCOR group in which R is as defined below,
R4 represents a hydrogen atom, a hydroxyl group, an OR group, an OCH2R group or an OCOR group, in which R is as defined below,
Y is a carbon, nitrogen or sulfur atom,
R5 and R6 each independently are absent or represent a hydrogen atom, a CH2 group or an R group as defined below, or else R5 and R6 together represent an oxygen or sulfur atom,
X represents a CH2 group, or an oxygen or sulfur atom,
m represents an integer equal to 0 or 1,
R represents a hydrogen atom or a saturated or unsaturated, branched or unbranched, cyclic or noncyclic hydrocarbon-based
chain which may or may not be substituted with a halogen group, and which carries polar or nonpolar functions,
n is an integer between 3 and 15, and
the substituents R1 to R5, R, X and Y and the integer m may be different in nature according to the units; and
c) detecting the presence of PrP.
|