US 7,402,441 B2
	Method of detecting an analyte in a fluid
Christopher Robin Lowe, Cambridge (United Kingdom); Colin Alexander Bennett Davidson, Cambridge (United Kingdom); Jeffrey Blyth, Cambridge (United Kingdom); Satyamoorthy Kabilan, Cambridge (United Kingdom); Alexander James Marshall, Cambridge (United Kingdom); Blanca Madrigal Gonzalez, Cambridge (United Kingdom); and Anthony Peter James, Cambridge (United Kingdom)
Assigned to Smart Holograms Limited, Cambridge (United Kingdom)
Appl. No. 10/509,782 PCT Filed Apr. 04, 2003, PCT No. PCT/GB03/01499 § 371(c)(1), (2), (4) Date Oct. 21, 2005, PCT Pub. No. WO03/087899, PCT Pub. Date Oct. 23, 2003.
Claims priority of application No. 0207944.0 (GB), filed on Apr. 05, 2002.
Prior Publication US 2006/0166350 A1, Jul. 27, 2006
Int. Cl. G01N 33/543 (2006.01)

U.S. Cl. 436—518  [422/55; 422/57; 422/58; 422/82.05; 422/82.11; 435/287.1; 435/287.2; 435/288.7; 435/808; 436/164; 436/165; 436/524; 436/527; 436/805]

14 Claims

1. A method for the detection of an analyte in a fluid, which comprises passing the fluid continuously over a holographic element comprising a medium and a hologram disposed throughout the volume of the medium, wherein an optical characteristic of the element changes as a result of a variation of a physical property occurring throughout the volume of the medium, wherein the variation arises as a result of interaction between the medium and the analyte, and wherein the reaction and the variation are reversible; and detecting any change of the optical characteristic.