| US 7,402,429 B1 | ||
| Host cells comprising cDNA corresponding to the antigenome of nonsegmented negative strand RNA viruses, further encoding antigenically active proteins | ||
| Martin A. Billeter, Zurich (Switzerland); Pius Spielhofer, Zurich (Switzerland); Karin Kälin, Gif-sur-Yvette (France); Frank Radecke, Zurich (Switzerland); and Henriette Schneider, Zurich (Switzerland) | ||
| Assigned to Schweiz Serum & Impfinstitut Bern, (Switzerland) | ||
| Appl. No. 9/11,425 PCT Filed Aug. 09, 1996, PCT No. PCT/EP96/03544 § 371(c)(1), (2), (4) Date Sep. 15, 1998, PCT Pub. No. WO97/06270, PCT Pub. Date Feb. 20, 1997. |
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| Claims priority of application No. 95112559 (EP), filed on Aug. 09, 1995. | ||
| Int. Cl. A01N 63/00 (2006.01); A01N 65/00 (2006.01); A61K 48/00 (2006.01); C12N 5/00 (2006.01); C12N 5/02 (2006.01); C12N 15/00 (2006.01) | ||
| U.S. Cl. 435—325 [435/320.1; 424/93.1; 424/93.2] | 20 Claims |
| 1. An isolated eukaryotic host cell comprising (1) stably transfected expression plasmids that allow genomic expression of
an exogenous RNA polymerase, a viral N protein, and a viral P protein in the host cell; (2) a nucleic acid sequence encoding
a viral L protein; and (3) a cDNA molecule, said cDNA molecule comprising:
(a) a (+) strand sequence of a non-segmented negative-strand RNA virus of the family Paramyxoviridae from which RNA transcripts
bearing the authentic 3′ termini can be transcribed; and
(b) an expression control sequence, wherein said (+) strand sequence of a non-segmented negative-strand RNA virus is operatively linked to the expression control
sequence, and wherein the host cell, without a replicating vaccinia helper virus, produces an infectious RNA virus containing
the (−) strand sequence of the non-segmented negative-strand RNA virus of the family Paramyxoviridae, said (−) strand sequence
of the non-segmented negative-strand RNA virus obeying the rule of six.
|