| CPC G06V 20/698 (2022.01) [G02B 21/367 (2013.01)] | 4 Claims |
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1. A high-power-microscope-assisted identification method of maize haploid plants, the method being implemented by a device comprising a high power microscope, a main frame disposed on an objective table of the high power microscope, and a computer, wherein the method comprises:
inputting sample information,
automatically testing samples in batches,
performing automatic analysis and comparison, and
automatically generating data results;
wherein a transfer box is disposed between the computer and the high power microscope; a data wire is electrically connected to an upper end of the transfer box, and a PLC, an image pickup unit, a processor, a memory, an analyzer and a buzzer are fixedly disposed inside the transfer box; a pluggable camera is fixedly disposed at a tail end of the data wire; and the pluggable camera is located at an eyepiece of the high power microscope;
wherein inputting sample information comprises:
selecting a reference sample of a haploid maize seed for growing a seedling, taking tissue from a root of the seedling and cutting the tissue into slices, placing one of the slices on the horizontal supporting plate along the L-shaped locating bar to be a locating point for testing, fixing the one slice by using the spring clip bars, and adjusting a focal length of the high power microscope;
controlling and adjusting parameters of movement time, movement locus and movement range of the horizontal actuator and the vertical actuator and inputting the parameters into the memory to be a testing procedure, and based on the testing procedure, starting the horizontal actuator and the vertical actuators by means of the processor and the PLC to drive the one slice to move along the movement locus which is fixed; and
collecting first images of different positions in the one slice of the reference sample by means of the camera and the image pickup unit, analyzing the first images of the different positions by the processor, inputting the first images to the memory, analyzing a number of chromosomes in the first images, the number is a comparative number, completing the inputting sample information, and restoring original positions of the horizontal supporting plate and the vertical supporting plate by the testing procedure after removing the one slice of the reference sample;
wherein automatically testing samples in batches comprises:
preparing slices of each of the samples to be tested based on: selecting one of the samples of a haploid maize seed for growing a seedling, taking tissue from a root of the seedling and cutting the tissue into slices, placing one of the slices on the horizontal supporting plate along the L-shaped locating bar to be a locating point for testing, fixing the one slice by using the spring clip bars, and adjusting a focal length of the high power microscope;
assigning respective labels to the slices;
testing the slices in sequence according to the respective labels: inputting one of the labels to the computer, placing one of the slices that corresponding to the one of the labels on the horizontal supporting plate, collecting images according to the testing procedure, and inputting collected first data into the memory in sequence; and
generating a warning sound by the buzzer after completing the testing procedure, performing next testing procedure, and completing sequential testing procedure of remaining ones of the slices with corresponding ones of the labels;
wherein performing automatic analysis and comparison comprises:
through the computer, extracting second images obtained in testing the slices in sequence according to the respective labels, and in generating a warning sound by the buzzer after completing the testing procedure, performing next test and identification, and completing sequential test and identification of remaining ones of the slices with corresponding ones of the labels, wherein each of the second images is corresponding to a corresponding one of the labels; and
analyzing the second images by the analyzer, calculating a number of chromosomes in the second images for comparison with the comparative number; comparing the number of chromosomes in the second images with the comparative number in the sample information;
identifying the samples to be tested as haploid plants when the number of chromosomes in the second images matches the comparative number, storing second data to the memory, and obtaining information indicating whether the maize haploid plants are haploids.
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