CPC G01N 21/65 (2013.01) [G01N 2201/1293 (2013.01)] | 29 Claims |
1. A method of quantifying viral titre in a viral culture using Raman spectroscopy, the method comprising the steps of:
(a) providing an unprocessed viral culture medium and irradiating the unprocessed viral culture medium with a light source;
(b) measuring the total intensity of Raman scattered light within each one of a plurality of wavenumber ranges to obtain a wavenumber intensity data set for the viral culture medium, wherein the plurality of wavenumber ranges are pre-selected and are characteristic of the virus in the viral culture medium;
(c) performing mathematical data processing steps on the wavenumber intensity data; and
(d) quantifying the viral titre based upon the output of the mathematical data processing steps.
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