US 11,703,455 B2
Methods for determining viral titre using raman spectroscopy
John Churchwell, London (GB); Marc Olivier Baradez, London (GB); and Damian Marshall, London (GB)
Assigned to CELL THERAPY CATAPULT LIMITED, London (GB)
Appl. No. 17/417,466
Filed by CELL THERAPY CATAPULT LIMITED, Great Maze Pond Greater London (GB)
PCT Filed Dec. 23, 2019, PCT No. PCT/GB2019/053695
§ 371(c)(1), (2) Date Jun. 23, 2021,
PCT Pub. No. WO2020/136376, PCT Pub. Date Jul. 2, 2020.
Claims priority of application No. 1821206 (GB), filed on Dec. 24, 2018.
Prior Publication US 2022/0018782 A1, Jan. 20, 2022
Int. Cl. G01J 3/44 (2006.01); G01N 21/65 (2006.01)
CPC G01N 21/65 (2013.01) [G01N 2201/1293 (2013.01)] 29 Claims
OG exemplary drawing
 
1. A method of quantifying viral titre in a viral culture using Raman spectroscopy, the method comprising the steps of:
(a) providing an unprocessed viral culture medium and irradiating the unprocessed viral culture medium with a light source;
(b) measuring the total intensity of Raman scattered light within each one of a plurality of wavenumber ranges to obtain a wavenumber intensity data set for the viral culture medium, wherein the plurality of wavenumber ranges are pre-selected and are characteristic of the virus in the viral culture medium;
(c) performing mathematical data processing steps on the wavenumber intensity data; and
(d) quantifying the viral titre based upon the output of the mathematical data processing steps.