US 11,703,454 B2
Method and apparatus for multiplexed imaging of spectrally-similar fluorophores
Jae-Byum Chang, Daejeon (KR); Young-Gyu Yoon, Daejeon (KR); Junyoung Seo, Daejeon (KR); and Yeonbo Sim, Daejeon (KR)
Assigned to Korea Advanced Institute of Science and Technology, Daejeon (KR)
Filed by Korea Advanced Institute of Science and Technology, Daejeon (KR)
Filed on Dec. 23, 2020, as Appl. No. 17/132,628.
Claims priority of application No. 10-2020-0000976 (KR), filed on Jan. 3, 2020; and application No. 10-2020-0088091 (KR), filed on Jul. 16, 2020.
Prior Publication US 2021/0208076 A1, Jul. 8, 2021
Int. Cl. G01N 21/64 (2006.01); G06T 7/00 (2017.01)
CPC G01N 21/6486 (2013.01) [G01N 21/6456 (2013.01); G06T 7/0012 (2013.01); G06T 2207/10064 (2013.01); G06T 2207/30024 (2013.01)] 20 Claims
OG exemplary drawing
 
1. An operating method of an electronic apparatus, said method comprising:
obtaining at least one image for a plurality of fluorophores labeled to different biomolecules respectively and
unmixing the obtained image into images for each of the biomolecules without using previously determined, collected, measured, or stored emission spectra of the fluorophores that were used to label the biomolecules,
wherein at least two of the fluorophores indicate corresponding first and second emission spectra of similar first and second spectral ranges, respectively and
wherein unmixing the obtained image comprises inferring a ratio of brightness of one of the at least two fluorophores in the first spectral range to said brightness in the second spectral range.