CPC C40B 50/06 (2013.01) [C07K 16/00 (2013.01); C12N 15/70 (2013.01); C07K 2317/24 (2013.01); C07K 2317/55 (2013.01); C07K 2317/622 (2013.01); C07K 2319/60 (2013.01)] | 13 Claims |
1. A method of creating a plasmid for use in producing a chimeric antibody, comprising:
(a) receiving a FAB region of the antibody;
(b) receiving a fluorescent protein;
(c) receiving a linker having length of at least 5 amino acids (natural or unnatural, synthetic);
(d) using the Gibson assembly process to join the FAB region, the fluorescent protein, and the linker into an expression plasmid.
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