US 11,702,766 B2
Systematic creation of fluorescent fusion polypeptides
Amanda Carroll-Portillo, Albuquerque, NM (US)
Filed by Sandia Biotech, Inc., Albuquerque, NM (US)
Filed on May 13, 2020, as Appl. No. 15/931,154.
Claims priority of provisional application 62/847,594, filed on May 14, 2019.
Prior Publication US 2021/0010009 A1, Jan. 14, 2021
Int. Cl. C40B 50/06 (2006.01); C12N 15/70 (2006.01); C07K 16/00 (2006.01)
CPC C40B 50/06 (2013.01) [C07K 16/00 (2013.01); C12N 15/70 (2013.01); C07K 2317/24 (2013.01); C07K 2317/55 (2013.01); C07K 2317/622 (2013.01); C07K 2319/60 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A method of creating a plasmid for use in producing a chimeric antibody, comprising:
(a) receiving a FAB region of the antibody;
(b) receiving a fluorescent protein;
(c) receiving a linker having length of at least 5 amino acids (natural or unnatural, synthetic);
(d) using the Gibson assembly process to join the FAB region, the fluorescent protein, and the linker into an expression plasmid.