US 11,702,671 B2
Importation of mitochondrial protein by an enhanced allotopic approach
Marisol Corral-Debrinski, Montreuil (FR); Jose-Alain Sahel, Paris (FR); Valerie Kaltimbacher, Fontenay Sous Bois (FR); and Crystel Bonnet, Montrouge (FR)
Assigned to INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM), Paris (FR)
Filed by INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM), Paris (FR)
Filed on May 26, 2021, as Appl. No. 17/331,097.
Application 16/574,685 is a division of application No. 15/914,566, filed on Mar. 7, 2018, abandoned.
Application 14/676,846 is a division of application No. 14/323,393, filed on Jul. 3, 2014, abandoned.
Application 14/323,393 is a division of application No. 11/913,618, granted, now 9,017,999, issued on Apr. 28, 2015, previously published as PCT/EP2006/005323, filed on May 3, 2006.
Application 17/331,097 is a continuation of application No. 16/574,685, filed on Sep. 18, 2019, abandoned.
Application 15/914,566 is a continuation of application No. 14/676,846, filed on Apr. 2, 2015, abandoned.
Claims priority of provisional application 60/676,933, filed on May 3, 2005.
Prior Publication US 2021/0292785 A1, Sep. 23, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/85 (2006.01); C12N 15/00 (2006.01); A61K 48/00 (2006.01); C12N 9/02 (2006.01); C07K 14/47 (2006.01); C12N 9/10 (2006.01)
CPC C12N 15/85 (2013.01) [A61K 48/005 (2013.01); A61K 48/0058 (2013.01); A61K 48/0066 (2013.01); C07K 14/47 (2013.01); C12N 9/0053 (2013.01); C12N 9/0089 (2013.01); C12N 9/1085 (2013.01); C12N 15/8509 (2013.01); C12Y 109/03001 (2013.01); C07K 2319/07 (2013.01); C12N 2800/22 (2013.01); C12N 2810/80 (2013.01)] 13 Claims
 
1. An expression vector for the efficient and stable delivery of mitochondrial protein ND4 into the mitochondrion of a mammalian cell, the vector comprising in the 5′ to 3′direction:
a mitochondrion-targeting nucleic acid sequence (MTS);
a nucleic acid sequence encoding mitochondrial ND4 protein; and
a 3′UTR nucleic acid sequence,
wherein the MTS comprises a nucleic acid sequence encoding the peptide consisting of the amino acid sequence as set forth in SEQ ID NO: 46;
wherein the nucleic acid sequence encoding mitochondrial ND4 protein is as set forth in SEQ ID NO: 29; and
wherein the 3′UTR nucleic acid sequence is selected from a group consisting of the nucleotide sequence as set forth in SEQ ID NO: 47, and nucleotides 1588 to 3010 as set forth in SEQ ID NO: 26.