CPC C12N 15/85 (2013.01) [A61K 48/005 (2013.01); A61K 48/0058 (2013.01); A61K 48/0066 (2013.01); C07K 14/47 (2013.01); C12N 9/0053 (2013.01); C12N 9/0089 (2013.01); C12N 9/1085 (2013.01); C12N 15/8509 (2013.01); C12Y 109/03001 (2013.01); C07K 2319/07 (2013.01); C12N 2800/22 (2013.01); C12N 2810/80 (2013.01)] | 13 Claims |
1. An expression vector for the efficient and stable delivery of mitochondrial protein ND4 into the mitochondrion of a mammalian cell, the vector comprising in the 5′ to 3′direction:
a mitochondrion-targeting nucleic acid sequence (MTS);
a nucleic acid sequence encoding mitochondrial ND4 protein; and
a 3′UTR nucleic acid sequence,
wherein the MTS comprises a nucleic acid sequence encoding the peptide consisting of the amino acid sequence as set forth in SEQ ID NO: 46;
wherein the nucleic acid sequence encoding mitochondrial ND4 protein is as set forth in SEQ ID NO: 29; and
wherein the 3′UTR nucleic acid sequence is selected from a group consisting of the nucleotide sequence as set forth in SEQ ID NO: 47, and nucleotides 1588 to 3010 as set forth in SEQ ID NO: 26.
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