	US 11,702,668 B2
	Plant regulatory elements and methods of use thereof
Andrew Carl Crow, Grimes, IA (US); Scott Diehn, West Des Moines, IA (US); and Lynne Eileen Sims, Polk City, IA (US)
Assigned to PIONEER HI-BRED INTERNATIONAL, INC.
Appl. No. 17/56,219
Filed by PIONEER HI-BRED INTERNATIONAL, INC., Johnston, IA (US)
PCT Filed May 20, 2019, PCT No. PCT/US2019/033040 § 371(c)(1), (2) Date Nov. 17, 2020, PCT Pub. No. WO2019/226508, PCT Pub. Date Nov. 28, 2019.
Claims priority of provisional application 62/674,994, filed on May 22, 2018.
Prior Publication US 2021/0230622 A1, Jul. 29, 2021
Int. Cl. C12N 15/82 (2006.01)

CPC C12N 15/8223 (2013.01) [C12N 15/823 (2013.01); C12N 15/8286 (2013.01)]

17 Claims

1. A DNA construct comprising a heterologous transcribable polynucleotide molecule operably linked to the regulatory element polynucleotide, wherein the regulatory element polynucleotide comprises:

a. a polynucleotide having at least 99% sequence identity to the nucleic acid sequence of any one of SEQ ID NO: 1 or 5-9;

b. a polynucleotide of any one of SEQ ID NO: 1 or 5-9; or

c. a functional fragment of at least 100 contiguous nucleotides of any one of SEQ ID NO: 1 or 5-9;

wherein the regulatory element polynucleotide has promoter activity.