US 11,702,658 B2
Methods and compositions for editing RNAs
Pengfei Yuan, Beijing (CN); Yanxia Zhao, Beijing (CN); Nengyin Liu, Beijing (CN); Zexuan Yi, Beijing (CN); Gangbin Tang, Beijing (CN); Wensheng Wei, Beijing (CN); Liang Qu, Beijing (CN); Zongyi Yi, Beijing (CN); Shiyou Zhu, Beijing (CN); Chunhui Wang, Beijing (CN); Zhongzheng Cao, Beijing (CN); and Zhuo Zhou, Beijing (CN)
Assigned to EDIGENE THERAPEUTICS (BEIJING) INC., Beijing (CN); and PEKING UNIVERSITY, Beijing (CN)
Filed by EDIGENE THERAPEUTICS (BEIJING) INC., Beijing (CN); and Peking University, Beijing (CN)
Filed on Oct. 14, 2021, as Appl. No. 17/501,954.
Application 17/501,954 is a continuation of application No. PCT/CN2020/084922, filed on Apr. 15, 2020.
Claims priority of application No. PCT/CN2019/129952 (WO), filed on Dec. 30, 2019; and application No. PCT/CN2019/082713 (WO), filed on Apr. 15, 2020.
Prior Publication US 2022/0098587 A1, Mar. 31, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/113 (2010.01); C12N 15/90 (2006.01); C12N 15/10 (2006.01)
CPC C12N 15/113 (2013.01) [C12N 15/102 (2013.01); C12N 15/907 (2013.01); C12N 2310/11 (2013.01); C12N 2310/16 (2013.01); C12N 2310/315 (2013.01); C12N 2310/321 (2013.01); C12N 2310/3519 (2013.01); C12N 2310/531 (2013.01); C12N 2310/533 (2013.01)] 16 Claims
 
1. A deaminase-recruiting RNA (dRNA) of about 60 to about 200 nucleotides, wherein:
a) the dRNA comprises a complementary RNA sequence capable of hybridizing to a target RNA, wherein the dRNA does not comprise an adenosine deaminases acting on RNA (ADAR)-recruiting domain capable of forming an intramolecular stem loop structure for binding an ADAR enzyme;
b) the dRNA is capable of recruiting a deaminase, a construct comprising a deaminase, or a construct comprising a catalytic domain of a deaminase, to deaminate a target adenosine in the target RNA; and
c) the dRNA comprises one or more chemical modifications;
wherein the complementary RNA sequence comprises a cytidine, an adenosine, or a uridine directly opposite to a target adenosine in the target RNA;
wherein the cytidine, adenosine, or uridine directly opposite to the target adenosine locates at least about 7 nucleotides away from the 3′ end of the complementary RNA sequence, and at least about 25 nucleotides away from the 5′ end of the complementary RNA sequence; and
wherein the length of the 5′ sequence within the complementary RNA sequence flanking the cytidine, adenosine, or uridine directly opposite to the target adenosine is longer than the length of the 3′ sequence within the complementary RNA sequence.