US 11,702,462 B2
Gene transfer systems for stem cell engineering
Arash Hatefi, Metuchen, NJ (US); Xuguang Chen, Somerset, NJ (US); and Alireza Nomani, New Brunswick, NJ (US)
Assigned to RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY, New Brunswick, NJ (US)
Appl. No. 16/631,309
Filed by Rutgers, The State University of New Jersey, New Brunswick, NJ (US)
PCT Filed Jul. 19, 2018, PCT No. PCT/US2018/042909
§ 371(c)(1), (2) Date Jan. 15, 2020,
PCT Pub. No. WO2019/018660, PCT Pub. Date Jan. 24, 2019.
Claims priority of provisional application 62/534,300, filed on Jul. 19, 2017.
Prior Publication US 2020/0207834 A1, Jul. 2, 2020
Int. Cl. C07K 14/81 (2006.01); A61K 47/62 (2017.01); C07K 14/00 (2006.01); C12P 21/02 (2006.01); C07K 14/71 (2006.01); C07K 14/435 (2006.01); C12N 5/0775 (2010.01); C12N 9/24 (2006.01); C12N 15/85 (2006.01); C07K 7/04 (2006.01); C12N 15/63 (2006.01); C12N 15/11 (2006.01)
CPC C07K 14/71 (2013.01) [C07K 7/04 (2013.01); C07K 14/001 (2013.01); C07K 14/435 (2013.01); C12N 5/0667 (2013.01); C12N 9/2497 (2013.01); C12N 15/63 (2013.01); C12N 15/85 (2013.01); C07K 2319/00 (2013.01); C07K 2319/01 (2013.01); C07K 2319/06 (2013.01); C07K 2319/74 (2013.01); C07K 2319/80 (2013.01); C12N 15/11 (2013.01); C12N 2500/25 (2013.01); C12N 2510/00 (2013.01)] 17 Claims
 
1. A fusion polypeptide, comprising:
i. a cell surface receptor-binding peptide, wherein the cell surface receptor-binding peptide is a vascular endothelial growth factor receptor (VEGFR)-binding peptide comprising an amino acid sequence at least 90% identical to the amino acid sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 2;
ii. a nucleic acid-binding polypeptide, wherein the nucleic acid-binding polypeptide comprises 2 to 10 repeats of a histone H2A comprising SEQ ID NO: 248 or SEQ ID NO: 5; and
iii. an endosomolytic peptide, wherein the endosomolytic peptide is GALA comprising and has an amino acid at least 90% identical to the amino acid sequence set forth in SEQ ID NO: 6.