US 11,701,657 B2
Column-based device for retrieval of rare cells based on size, and uses thereof
Jackie Y. Ying, Singapore (SG); Min-Han Tan, Singapore (SG); Yoke San Daniel Lee, Singapore (SG); Igor Cima, Singapore (SG); Yeon Joon Park, Singapore (SG); and Wai Min Phyo, Singapore (SG)
Assigned to AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH, Singapore (SG)
Filed by Agency for Science, Technology and Research, Singapore (SG)
Filed on Oct. 22, 2020, as Appl. No. 17/77,828.
Application 17/077,828 is a division of application No. 15/544,761, granted, now 11,084,034, previously published as PCT/SG2016/050027, filed on Jan. 21, 2016.
Claims priority of application No. 10201500471Q (SG), filed on Jan. 21, 2015; and application No. 10201500472R (SG), filed on Jan. 21, 2015.
Prior Publication US 2021/0106997 A1, Apr. 15, 2021
Int. Cl. B01L 3/00 (2006.01); C12N 5/071 (2010.01); G01N 33/50 (2006.01); G01N 33/574 (2006.01); C12Q 1/6886 (2018.01)
CPC B01L 3/502761 (2013.01) [C12N 5/069 (2013.01); C12Q 1/6886 (2013.01); G01N 33/5064 (2013.01); G01N 33/5094 (2013.01); G01N 33/574 (2013.01); G01N 33/57419 (2013.01); G01N 33/57492 (2013.01); B01L 2200/027 (2013.01); B01L 2200/0647 (2013.01); B01L 2300/042 (2013.01); B01L 2300/0681 (2013.01); C12Q 2600/156 (2013.01)] 12 Claims
OG exemplary drawing
 
1. An apparatus for capturing and retrieving a cell from a sample, comprising at least one column, the column comprising:
(i) an inner wall defining an inner chamber, the inner chamber having an inlet opening at a first end of the column for receiving the sample, and an outlet opening at a second end of the column;
(ii) a perforated plug disposed within the inner chamber adjacent to the second end of the column;
(iii) a sleeve insert having an opening at a first end and an opening at a second end, the sleeve insert comprising a channel tapered at the second end and disposed within the inner chamber with its second end adjacent to the perforated plug; and
(iv) a filtering means housed within the sleeve insert, the filtering means comprising a sieve sandwiched between two sealing means.