CPC A61K 48/0075 (2013.01) [A61K 47/6901 (2017.08); A61N 7/00 (2013.01); C12N 15/86 (2013.01); A61K 9/7023 (2013.01); C12N 2750/14143 (2013.01); C12N 2750/14145 (2013.01); C12N 2750/14151 (2013.01); C12N 2810/859 (2013.01)] | 34 Claims |
1. A method of systemic delivery of a polypeptide to a subject comprising genetically modifying target skin cells within skin of a subject comprising
administering to the subject an engineered virus comprising one or more foreign nucleic acid sequences encoding one or more target polypeptides, wherein the engineered virus is an adeno-associated viral vector,
wherein the one or more foreign nucleic acid sequences of the engineered virus are introduced into the target skin cells within the skin to produce genetically modified skin cells, and wherein the genetically modified skin cells produce the one or more target polypeptides by expression of the one or more foreign nucleic acid sequences, and
wherein the one or more target polypeptides are excreted from the genetically modified skin cells and are introduced systemically within the subject.
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