	US 11,701,389 B2
	Methods of preserving mesenchymal stem cells
Jacques Galipeau, Madison, WI (US); and Raghavan Chinnadurai, Tucker, GA (US)
Assigned to Emory University, Atlanta, GA (US); and Children's Healthcare of Atlanta, Inc., Atlanta, GA (US)
Appl. No. 16/83,661
Filed by Emory University, Atlanta, GA (US); and Children's Healthcare of Atlanta, Inc., Atlanta, GA (US)
PCT Filed Mar. 9, 2017, PCT No. PCT/US2017/021523 § 371(c)(1), (2) Date Sep. 10, 2018, PCT Pub. No. WO2017/156235, PCT Pub. Date Sep. 14, 2017.
Claims priority of provisional application 62/305,644, filed on Mar. 9, 2016.
Prior Publication US 2019/0099449 A1, Apr. 4, 2019
Int. Cl. A61K 35/28 (2015.01); A01N 1/02 (2006.01); C12N 5/0775 (2010.01)

CPC A61K 35/28 (2013.01) [A01N 1/0215 (2013.01); A01N 1/0221 (2013.01); A01N 1/0226 (2013.01); A01N 1/0284 (2013.01); C12N 5/0663 (2013.01); C12N 2501/24 (2013.01); C12N 2523/00 (2013.01)]

8 Claims

1. A method of cryopreserving mesenchymal stem cells comprising

mixing mesenchymal stem cells with interferon gamma at a concentration of 20 ng/mL in culture medium for 48 hours providing mixed mesenchymal stem cells, and cooling the mixed mesenchymal stem cells to a temperature below zero degrees Celsius providing frozen interferon gamma exposed mesenchymal stem cells, wherein mixing is prior to cooling the mixed mesenchymal stem cells, and

thawing the frozen interferon gamma exposed mesenchymal stem cells to a temperature above zero degrees Celsius providing thawed interferon gamma exposed mesenchymal stem cells that express indoleamine 2,3-dioxygenase (IDO) mRNA with greater than a 100-fold change in concentration when compared to cryopreserved mesenchymal stem cells that were not mixed with interferon gamma prior to freezing.