US 7,563,623 B2
Biosensor
Toshihide Ezoe, Kanagawa (Japan); Taisei Nishimi, Kanagawa (Japan); and Yukou Saito, Kanagawa (Japan)
Assigned to FUJIFILM Corporation, Tokyo (Japan)
Filed on Feb. 21, 2007, as Appl. No. 11/708,637.
Claims priority of application No. 2006-045615 (JP), filed on Feb. 22, 2006.
Prior Publication US 2008/0038841 A1, Feb. 14, 2008
Int. Cl. G01N 33/551 (2006.01)
U.S. Cl. 436—524  [385/12; 385/129; 385/130; 422/57; 422/82.11; 427/2.11; 427/2.13; 427/162; 427/163.2; 427/240; 427/241; 427/421.1; 435/287.2; 435/288.7; 435/808; 436/164; 436/525; 436/805] 18 Claims
 
1. A method for producing a biosensor comprising a substrate on which a hydrophilic polymer having a reactive group capable of binding to a physiologically active substance is immobilized via a compound represented by the following formula (1), said compound at a concentration of 1 mM being dissolved in water at 25° C.:
X-L-Y  (1)
wherein X represents a group capable of binding to metal, L represents a linking group, and Y represents a functional group to which the hydrophilic polymer is bound, which comprises a step of coating the substrate with a solution of the compound represented by formula (1) in water, and a step of allowing the hydrophilic polymer having a reactive group capable of binding to a physiologically active substance to come into contact with the substrate coated with the solution of the compound represented by formula (1), thereby allowing the hydrophilic polymer to bind to the compound represented by formula (1).