	US 7,563,594 B2
	Cytolethal distending toxins and detection of Campylobacter bacteria using the same as a target
Shinji Yamasaki, Osaka (Japan); and Masahiro Asakura, Osaka (Japan)
Assigned to Fuso Pharmaceutical Industries, Ltd., Osaka (Japan)
Appl. No. 10/581,757 PCT Filed Dec. 03, 2004, PCT No. PCT/JP2004/018042 § 371(c)(1), (2), (4) Date Mar. 02, 2007, PCT Pub. No. WO2005/054472, PCT Pub. Date Jun. 16, 2005.
Claims priority of application No. 2003-408103 (JP), filed on Dec. 05, 2003.
Prior Publication US 2007/0212691 A1, Sep. 13, 2007
Int. Cl. C12P 21/06 (2006.01); C07H 17/00 (2006.01)

U.S. Cl. 435—69.1 [435/320.1; 435/252.3; 435/325; 536/23.1]

4 Claims

1. An isolated polynucleotide encoding a cytolethal distending toxin, which is any one of:

(a) a polynucleotide encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 2;

(b) a polynucleotide comprising the nucleotide sequence of position 1 to 777 in the nucleotide sequence of SEQ ID NO: 1;

(c) a polynucleotide encoding a polypeptide comprising an amino acid sequence with a substitution, deletion, addition, and/or insertion of one to 30 amino acids in the amino acid sequence of SEQ ID NO:2;

(d) a polynucleotide that hybridizes under a stringent condition of 1×SSC, 0.1% SDS, and 37° C. to DNA comprising the nucleotide sequence of position 1 to 777 in the nucleotide sequence of SEQ ID NO: 1.