| US 7,563,580 B2 | ||
| Nucleic acid adsorbent; methods for adsorbing, removing, dissociating, and recovering nucleic acids using the same; and method for regenerating the same | ||
| Chuichi Hirayama, Kmamoto (Japan); Masayo Sakata, Kumamoto (Japan); Masami Todokoro, Tokyo (Japan); and Minoru Nakayama, Minamata (Japan) | ||
| Assigned to Chisso Corporation, Osaka (Japan); and Kumamoto University, Kumamoto (Japan) | ||
| Filed on Feb. 27, 2008, as Appl. No. 12/71,891. | ||
| Application 12/071891 is a division of application No. 11/214773, filed on Aug. 31, 2005, granted, now 7,358,050. | ||
| Claims priority of application No. 2004-252114 (JP), filed on Aug. 31, 2004; and application No. 2005-243460 (JP), filed on Aug. 24, 2005. | ||
| Prior Publication US 2008/0227969 A1, Sep. 18, 2008 | ||
| Int. Cl. C12Q 1/68 (2006.01); C07H 21/00 (2006.01) | ||
| U.S. Cl. 435—6 [435/5; 435/7.1; 435/7.2; 536/23.1] | 17 Claims |
| 1. A method for removing a nucleic acid contained in cell extract, comprising:
mixing a nucleic acid adsorbent with a first solution containing the nucleic acid, the nucleic acid adsorbent comprising a
polymer immobilized on a water-insoluble carrier; and
separating from the cell extract, the nucleic acid adsorbent that has adsorbed the nucleic acid,
wherein the constitutional unit of the polymer is represented by the following formula (I):
 wherein R1 represents hydrogen, fluoride, or methyl; R2 and R3 each independently represent alkyl of 1 to 6 carbon atoms; A represents —NH— or —O—; and B represents alkylene of 2 to 18
carbon atoms.
|