| US 7,556,948 B2 | ||
| Method for producing crystallized pleuromutilins | ||
| Michael John Rees, Harlow (United Kingdom); Keith Graham Robins, Worthing (United Kingdom); Anna Louisa Stefanska, Harlow (United Kingdom); Jan Edward Thirkettle, Brentford (United Kingdom); Michael Sidney Verrall, East Grinstead (United Kingdom); and David Alan Yeandle, Worthing (United Kingdom) | ||
| Assigned to Glaxo Group Limited, Greenford, Middlesex (United Kingdom) | ||
| Appl. No. 10/524,099 PCT Filed Aug. 07, 2003, PCT No. PCT/GB03/03452 § 371(c)(1), (2), (4) Date Oct. 07, 2005, PCT Pub. No. WO2004/015122, PCT Pub. Date Feb. 19, 2004. |
||
| Claims priority of application No. 0218578.3 (GB), filed on Aug. 09, 2002. | ||
| Prior Publication US 2006/0166341 A1, Jul. 27, 2006 | ||
| Int. Cl. A23K 1/17 (2006.01); C12P 19/44 (2006.01) | ||
| U.S. Cl. 435—117 [435/118; 435/119; 435/127; 435/77] | 19 Claims |
| 1. A method for preparing one or more crystallised pleuromutilins comprising the steps of:
a) culturing a pleuromutilins-producing microorganism in a liquid culture medium;
b) extracting pleuromutilins from the unfiltered culture medium with a water immiscible organic solvent which is 4-methyl-2-pentanone
(MIBK);
c) concentrating the extracted pleuromutilins;
d) directly crystallizing the pleuromutilins from MIBK using a miscible non polar solvent which is heptane.
|